pSKAN8 vector,lyophilized DNA
Opis
pSKAN8 is a phagemid vector used to prepare your own phagemid display library.
Note: The system is designed for the presentation of a hypervariable amino acid loop of 6 - 8 amino acids. We have no experience with inserting larger loops. Thus, this will have to be determined empirically.
Phagemid vector construction.
pSKAN8 is based on the vector pMAMPF-3. Between the two Cys a hypervariable amino acid loop (with (NNG/T)6-8) can be created using the protocol in chapter (See also the ready-to-use pSKAN libraries.)
The phagemid contains the genetic elements:
- AmpR: selection marker (ampicillin resistance)
- ColE1 ori: plasmid origin of replication
- fd ori: origin of replication for the fd phage
- λpL: λ-promoter left
- ompA: signal sequence for secretion
- hPSTI: gene of the human pancreatic secretory trypsin inhibitor
- pIII: gene for the phage coat protein III
- 2 x tfd: two phage fd transcription terminator sequences
- catamber: chloramphenicol resistance is inactive due to an amber mutation
Dane techniczne
Opakowanie | 5 µg |