Produkty
Producenci

pSKAN8 vector,lyophilized DNA

nr kat.: PSKAN8
Opakowanie: 5 µg
Cena brutto: do wyceny
ilość szt.

towar niedostępny

dodaj do przechowalni

Opis

pSKAN8 is a phagemid vector used to prepare your own phagemid display library.

Note: The system is designed for the presentation of a hypervariable amino acid loop of 6 - 8 amino acids. We have no experience with inserting larger loops. Thus, this will have to be determined empirically.

Phagemid vector construction.
pSKAN8 is based on the vector pMAMPF-3. Between the two Cys a hypervariable amino acid loop (with (NNG/T)6-8) can be created using the protocol in chapter (See also the ready-to-use pSKAN libraries.)

The phagemid contains the genetic elements:

  • AmpR: selection marker (ampicillin resistance)
  • ColE1 ori: plasmid origin of replication
  • fd ori: origin of replication for the fd phage
  • λpL: λ-promoter left
  • ompA: signal sequence for secretion
  • hPSTI: gene of the human pancreatic secretory trypsin inhibitor
  • pIII: gene for the phage coat protein III
  • 2 x tfd: two phage fd transcription terminator sequences
  • catamber: chloramphenicol resistance is inactive due to an amber mutation

 

Dane techniczne

Opakowanie 5 µg
do góry
Sklep jest w trybie podglądu
Pokaż pełną wersję strony
Sklep internetowy Shoper.pl