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pEG-His 1 vector

Cena brutto: 303,07 €
Cena netto: 246,40 €
szt.
Opakowanie:
5 µg
Kod produktu:

PEG01

Producent:
Pliki do pobrania:

The pEG-His1 vector was constructed for the expression of toxic gene products in E.coli. To obtain its exceptional tightness prior induction with IPTG, the lac I repressor gene has been included in the vector and is overexpressed in plasmid bearing cells.

Recombinant fusion proteins with a 6xHis tag can be easily and selectively purified using the unique Ni-NTA (nickel-nitrilotriacetic acid) technology. The Ni-NTA affinity matrix shows an optimal binding capacity and a minimized non-specific binding resulting in highly purified and reproducible 6xHis-tagged protein preparations.

Features:

  • optimized promoter guarantees excellent expression levels
  • very tight expression control due to overexpression of the Lac I repressor
  • a convenient MCS allows flexible and easy cloning of the insert
  • start codon is provided by an Nde I site
  • inserts can be expressed as C-terminal tagged 6xHis fusion proteins for efficient and easy one-step purification of full length proteins by metal-chelate affinity chromatography
  • an RGS motive and the proximate Poly(His)-Tag allow detection and/or immunoprecipitation of the expressed protein with commercial anti-RGS and anti-His antibodies

 

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