Produkty
Producenci

anti P III - antibody, monoclonal mouse, lyophilized

nr kat.: PSKAN3
Opakowanie: 100 µl
Cena brutto: do wyceny
ilość szt.

towar niedostępny

dodaj do przechowalni

Opis

Features:

  • Antibody to phage M13 coat protein g3p (pIII)
  • Clone: 10C3
  • Monoclonal mouse IgG

 

The anti-g3p antibody is a useful analytical tool for current phage display systems using the g3-protein of M13 for display of recombinant proteins such as antibodies, protein- or peptide-libraries and more. (See also our pSKAN Phagemid Display System).

Applications:
The antibody can be used in Western blots in order to analyze:

  • Correct reading-frame fusions of g3p-fusion proteins
  • Expression levels of g3p-fusion proteins in E. coli and on the tip of the phage
  • Ratios of displayed g3p-proteins and g3p-fusion proteins on the phage-capsid
  • M13-infected E. coli strains

 

The epitope is also recognized under non-denaturing conditions. Therefore, the antibody can be used for:

  • ELISA detection of g3p-fusion proteins
  • Purification of g3p-fusion proteins by affinity chromatography
  • Efficient and rapid analysis of antigen binding characteristics of recombinant antibodies selected by phage display (microplate assay)

 

The Epitope:
The antigenic epitope of the anti-g3p antibody resides within amino acids 292 to 302 of the mature g3-protein and is therefore compatible with most of the phagemid vectors using different N-terminal deletions of the g3-protein.

 

Amino acid sequence of the epitope:

- A T D Y G A A I D G F -

Dane techniczne

Opakowanie 100 µl
do góry
Sklep jest w trybie podglądu
Pokaż pełną wersję strony
Sklep internetowy Shoper.pl