anti P III - antibody, monoclonal mouse, lyophilized
Opis
Features:
- Antibody to phage M13 coat protein g3p (pIII)
- Clone: 10C3
- Monoclonal mouse IgG
The anti-g3p antibody is a useful analytical tool for current phage display systems using the g3-protein of M13 for display of recombinant proteins such as antibodies, protein- or peptide-libraries and more. (See also our pSKAN Phagemid Display System).
Applications:
The antibody can be used in Western blots in order to analyze:
- Correct reading-frame fusions of g3p-fusion proteins
- Expression levels of g3p-fusion proteins in E. coli and on the tip of the phage
- Ratios of displayed g3p-proteins and g3p-fusion proteins on the phage-capsid
- M13-infected E. coli strains
The epitope is also recognized under non-denaturing conditions. Therefore, the antibody can be used for:
- ELISA detection of g3p-fusion proteins
- Purification of g3p-fusion proteins by affinity chromatography
- Efficient and rapid analysis of antigen binding characteristics of recombinant antibodies selected by phage display (microplate assay)
The Epitope:
The antigenic epitope of the anti-g3p antibody resides within amino acids 292 to 302 of the mature g3-protein and is therefore compatible with most of the phagemid vectors using different N-terminal deletions of the g3-protein.
Amino acid sequence of the epitope:
- A T D Y G A A I D G F -
Dane techniczne
Opakowanie | 100 µl |