anti-HIS-Tag antibody

Anti-His and Anti-GST Antibodies

Features:

• Optimally suited for monitoring expression of His-tagged and GST-tagged fusion proteins
• Useful for immunoaffinity purification of fusion proteins possessing a (His)₆ or GST tag
• Source: rabbit, polyclonal
• Markedly increased sensitivity for (His)6 and GST tags

Vectors for expression of fusion proteins possessing a (His)₆ tag or glutathione-S-transferase (GST) tag are commonly used for simple immunoaffinity purification of recombinant proteins produced in E. coli (e.g. by metal-chelate affinity chromatography). The MoBiTec anti-His-Tag and anti-GST-Tag antibodies are very useful in monitoring (His)₆ -Tag and GST-Tag fusion protein expression and purification and show a markedly increased sensitivity for (His)₆ tags and GST tags compared with other commercially available anti-His-Tag or anti-GST-Tag antibodies.

Antigen: KLH-Gly-Ser-(His)₆

Source: rabbit, polyclonal

Specificity: C-or N-terminal (His)₆–Tag

Contents: supplied as 100 ul serum

Recommended dilution: The antibody can be used for Western blottin at a 1:1,000 dilution. Immunoprecipitation and other immunological applications have not been tested so far.