Tris-Glycine (TG) Buffer 10× (liquid)
Opis
Tris-glycine buffer (TG) is the most common running buffer in native (non-denaturing) homogeneous and gradient poly-acrylamide gel electrophoresis (PAGE) of proteins. Tris-glycine gels resolve proteins by size. However, very small proteins and peptides do not resolve well due to interference from the glycine/pH discontinuity front. TG buffer is also used to make Tris-glycine/20% methanol Western transfer buffer, which is the most frequently used protein transfer buffer for wet blot transfers.
Features:
– Running buffers for protein electrophoresis
– Molecular Biology Grade
Applications:
– Protein electrophoresis
– Polyacrylamide gel electrophoresis
– Western blotting
Specifications:
– Molecular Biology Grade
– Each liter of concentrate makes 10 L of 1x Tris-glycine buffer
– DNase: none detected
– RNase: none detected
– Protease: none detected
– pH of diluted (1x) solution at 25 °C: 8.3-8.7
Storage: Store at room temperature.
Dane techniczne
Opakowanie | 1 l |