Description: Topoisomerase I (E. coli) catalyses the relaxation of negatively supercoiled DNA as the enzyme has been implicated in the knotting and unknotting double-stranded DNA. In addition, the enzyme is involved in the linking of complementary rings of single-stranded DNA into double-stranded rings.
Features:
– Promotes the relaxation of negatively-supercoiled DNA
– Isolated from a recombinant source
– Supplied with a highly optimized 10x reaction buffer
Applications:
– Relaxing negatively-supercoiled DNA
Specifications:
| Enzyme concentration: | 5 U/μL |
| Source: | Recombinant E. coli strain |
| Optimal reaction temperature: | 37 °C |
| Heat inactivation: | 65 °C for 20 min |
| Storage conditions: | Store at -20 °C |
| Shipping conditions: | Shipped at dry ice |
Components:
– Topoisomerase I (5 U/μL)
– Reaction Buffer (10x)