TEV Protease (TurboTEV), GST-& His-Tag

Description
MobiTEV Protease2 (TurboTEV) recombinant contains an enhanced form of a catalytic fragment of the N1a protein of Tobacco etch virus (TEV), a cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Gln and Gly.

MobiTEV Protease2 (short: MobiTEV2) is a restriction grade protease that has a robust activity at 4 °C with high specificity and great stability. It does not require any special buffer for its activity and can be used in a buffer most suitable for the target protein.

MobiTEV2 is a 52 kDa protein with both GST and His tags so it can be easily removed by either Ni-chelating or Glutathione (GSH) resin along with the cleaved tag.

Formulation & Storage
MobiTEV Protease2 (TurboTEV): 1 mg (10’000 units)/ 0.5 ml in 25 mM Tris-HCl, pH 8.0, 50 mM NaCl, 1 mM TCEP, 50% glycerol. Store MobiTEV Protease2 at -20 °C. The protease retains >80% activity after storage at room temperature for over 65 hours.

Activity and Specificity
MobiTEV2 (TurboTEV) is a 52 kDa protein. It has a specific activity of at least 10’000 units/mg, using the conventionally defined activity unit (1 unit cleaves >85% of 3 μg control substrate in 1 hour at 30 °C). In practice, 1 mg (10’000 units) of MobiTEV2 cleaves >90% of 100 mg of a control target protein at 4 °C in 16 hours.

No non-specific cleavage has been observed under the same condition when MobiTEV2 and the control target protein was mixed at 1:10 ratio.