Produkty
Producenci
T7 DNA Polymerase
nr kat.:
EP0081
Opakowanie:
300 U
Producent:
nr kat.:
EP0081
Opis
Concentration: 10u/µl
Thermo Scientific T7 DNA Polymerase, a template dependent DNA polymerase, catalyzes DNA synthesis in the 5'=>3' direction. It is a highly processive DNA polymerase allowing continuous synthesis of long stretches of DNA. The enzyme also exhibits a high 3'=>5' exonuclease activity towards single- and double-stranded DNA.
Highlights
Strong 3’=>5’ exonuclease activity, approximately 1000-fold greater than Klenow Fragment
Active in restriction enzyme buffers
Applications
- Purification of covalently closed circular DNA by removal of residual genomic DNA
- Primer extension reactions on long templates
- DNA 3'-end labeling
- Strand extensions in site-directed mutagenesis
- Fill-in blunting of 5'-overhang DNA
- Second strand synthesis of cDNA
- In situ detection of DNA fragmentation associated with apoptosis
Note
Assays at 37°C require only short incubation times.
Dane techniczne
| Opakowanie | 300 U |