T4 DNA ligase
Opis
T4 DNA Ligase is an ultrapure recombinant enzyme purified from Escherichia coli supplied with an optimized 10× Reaction Buffer, which includes ATP. T4 DNA Ligase catalyses the formation of a phosphodiester bond between juxtaposed 5′-phosphoryl and 3′-hydroxyl termini in duplex DNA. It repairs single-strand nicks in duplex DNA and will join both blunt and cohesive-end restriction fragments of duplex DNA or RNA. The enzyme requires ATP as cofactor.
Features:
Free from detectable nonspecific nuclease, endonuclease, RNase and DNase activities
Supplied with an optimized reaction buffer for efficient ligation reactions
Applications:
- Cloning of both blunt- and sticky (cohesive)-end restriction fragments
- Cloning of PCR products
- Joining linkers and adapters to DNA
- Nick repair
- Self-circularization of linear DNA
Specifications:
- Enzyme concentration: 5 U/μL
- Source: Recombinant E. coli strain
- Optimal reaction temperature: 16-20 °C
- Heat inactivation: 65 °C for 10 min
- Storage conditions: Store at -20 °C
- Shipping conditions: Shipped at dry ice
Components:
- T4 DNA Ligase (5 U/μL)
- Reaction buffer (10x)
EL0011, EL0014, EL0016
Dane techniczne
Opakowanie | 500 U |