Description: Supreme NZYTaq II 2× Colourless Master Mix is a premixed ready-to-use solution containing Supreme NZYTaq II DNA polymerase (MB355), a robust DNA polymerase derived from Taq DNA polymerase that was engineered to exhibit high processivity and to provide high PCR sensitivity. The enzyme, displaying, a hot-start-like PCR capacity, is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer-dimers and providing a highly specific DNA amplification. The functional activity of the enzyme is restored during a short 5-minute incubation step at 95 °C. In addition, the master mix contains dNTPs, reaction buffer and additives at optimal concentrations for the efficient amplification of a wide range of DNA templates of up to 6 kb. MgCl2 final concentration is 2.5 mM, allowing the implementation of a variety of PCR protocols.
Resulting PCR products have an A-overhang and are suitable for cloning with NZYTech´s NZY-A PCR cloning kit (MB053) or NZY-A Speedy PCR cloning kit (MB137).
Features:
– Convenient ready-to-use PCR master mix
– High sensitivity
– Eliminates nonspecific amplifications and minimizes primer-dimers
– Robust amplification using a wide range of DNA templates
– Leaves an A-overhang
Applications:
– Powerful PCR
– Colony PCR
– Generation of products for TA cloning
Specifications:
| Product length: | 0-6 kb |
| Hot-start-like capacity: | yes |
| Extension time: | 15-30 sec/kb at 72 °C |
| 3´→5´ activity: | no |
| Product overhang: | 3’-A |
| Storage conditions: | Store at -20 ºC |
| Shipping conditions: | Shipped at 4 ºC to dry ice |
Components:
Supreme NZYTaq II 2× Colourless Master Mix (0.2 U/μL)