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Supreme NZYProof 2x Colourless Master Mix

nr kat.: MB28602
Opakowanie: 200 react.
Cena brutto: 869,73 zł 869.73
Cena netto: 707,10 zł
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Producent: NZYTech
nr kat.: MB28602

Opis

200 reakcji x 50 μL

Description: Supreme NZYProof 2× Colourless Master Mix is a premixed ready-to-use solution containing Supreme NZYProof DNA polymerase (MB283), an engineered highly accurate, fast and sensitive variant of NZYProof DNA polymerase displaying a hot-start like PCR capacity.  This feature is achieved by a novel hot-start technology, which inhibits both polymerase and 3’→5’ exonuclease activities and thus avoids extension of non-specifically annealed primers or primer-dimers, as well as the degradation of primers and template DNA during PCR reaction setup. Thus, Supreme NZYProof DNA polymerase generates higher specificity, sensitivity and yield during the accurate amplification of DNA. The master mix contains dNTPs, reaction buffer and additives at optimal concentrations for the efficient amplification of DNA templates by PCR. Mg2+ final concentration is 1.5 mM, allowing the implementation of a variety of PCR protocols.

Supreme NZYProof DNA polymerase possesses 3´→5´ exonuclease proofreading activity. Resulting PCR products have blunt-ends and are suitable for cloning with NZYTech´s NZY-blunt PCR cloning kit (MB121).

 Features:
– Convenient ready-to-use PCR master mix
– Hot-start like capacity
– Proofreading activity

Applications:
– High-fidelity PCR
– Hot-start PCR
– Routine PCR
– Generation of products for blunt-end cloning

Specifications:

Product length: 0-10 kb
Hot-start like capacity: yes
Extension time: 15-30 sec/kb at 72 °C
3´→5´ activity: yes
Product overhang: blunt
Storage conditions: Store at -20 ºC
Shipping conditions: Shipped at 4 ºC to dry ice

Components:
Supreme NZYProof 2× Colourless Master Mix (0.2 U/μL)

Dane techniczne

Opakowanie 200 react.

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