Speedy Supreme NZYTaq 2x Green Master Mix
Opis
Speedy Supreme NZYTaq 2× Green Master Mix is a premixed ready-to-use solution containing Speedy Supreme NZYTaq DNA polymerase (MB390), a recombinant modified form of Taq DNA polymerase that combines hot-start like PCR capacity and a fast polymerization reaction. The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer-dimers and thus providing higher specificity and sensitivity of DNA amplification. The functional activity of the enzyme is restored during a short 5- minute incubation step at 95 °C. The master mix contains dNTPs, reaction buffer and additives at optimal concentrations for the efficient amplification of a wide range of DNA templates. MgCl2 final concentration is 2.5 mM, allowing the implementation of a variety of PCR protocols. In addition, reactions assembled with Speedy Supreme NZYTaq 2× Green Master Mix may be directly loaded onto agarose gels. The mix contains two dyes (blue and yellow) that allow monitoring the progress of the electrophoresis.
Resulting PCR products have an A-overhang and are suitable for cloning with NZYTech´s NZY-A PCR cloning kit (MB053) or NZY-A Speedy PCR cloning kit (MB137).
Features:
– Convenient ready-to-use PCR master mix
– Direct Gel Loading
– Fast routine amplification (5 sec/kb for DNA fragments up to 2-3 kb)
– Eliminates nonspecific amplifications and minimizes primer-dimers
– Robust amplification using a wide range of DNA templates
– Leaves an A-overhang
Applications:
– Fast PCR
– Powerful PCR
– Colony PCR
– Generation of products for TA cloning
Specifications:
Product length: | 0-6 kb |
Hot-start-like capacity: | yes |
Extension time: | 5 sec/kb (or 10 sec/kb for targets higher than 2-3 kb in size) at 72 °C |
3´→5´ activity: | no |
Product overhang: | 3’-A |
Storage conditions: | Store at -20 ºC |
Shipping conditions: | Shipped at 4 ºC to dry ice |
Components:
Speedy Supreme NZYTaq 2× Green Master Mix (0.2 U/μL)
Dane techniczne
Opakowanie | 1000 react. |