Speedy Supreme NZYTaq 2x Colourless Master Mix

Description: Speedy Supreme NZYTaq 2× Colourless Master Mix is a premixed ready-to-use solution containing Speedy Supreme NZYTaq DNA polymerase (MB390), a recombinant modified form of Taq DNA polymerase that combines hot-start like PCR capacity and a fast polymerization reaction. The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer-dimers and thus providing higher specificity and sensitivity of DNA amplification. The functional activity of the enzyme is restored during a short 5- minute incubation step at 95 °C. The master mix contains dNTPs, reaction buffer and additives at optimal concentrations for the efficient amplification of a wide range of DNA templates. MgCl₂ final concentration is 2.5 mM, allowing the implementation of a variety of PCR protocols.

Resulting PCR products have an A-overhang and are suitable for cloning with NZYTech´s NZY-A PCR cloning kit (MB053) or NZY-A Speedy PCR cloning kit (MB137).

Features:
– Convenient ready-to-use PCR master mix
– Fast routine amplification (5 sec/kb for DNA fragments up to 2-3 kb)
– Eliminates nonspecific amplifications and minimizes primer-dimers
– Robust amplification using a wide range of DNA templates
– Leaves an A-overhang

Applications:
– Fast PCR
– Powerful PCR
– Colony PCR
– Generation of products for TA cloning

Excellent performance of Speedy Supreme NZYTaq 2x Colourless Master Mix in fast amplification of diferent-sized DNA fragments
The performance of Speedy Supreme NZYTaq 2x Colourless Master Mix was tested in a fast PCR assay to amplify fragments of 1 kb, 2.5 kb and 5kb from diferent dilutions of human genomic DNA.

The protocol provided with the NZYTech’s Master Mix was followed using a limited extension period of 5 sec/kb to amplify the 1 kb fragment and 10 sec/kb to amplify both 2.5 kb and 5 kb. A 5-fold human genomic DNA dilution, ranging from 20 to 0.032 ng, was used for the amplification of  diferent-sized fragments. Lane M: NZYDNA Ladder III. PCRs were performed in 50 µL reaction mixtures with elongation times of 5 sec, 25 sec, and 50 sec for 1kb, 2.5 kb and 5 kb fragments, respectively. Results show successful amplification in conditions under test.

Specifications:

Components:
Speedy Supreme NZYTaq 2× Colourless Master Mix (0.2 U/μL)