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Speedy NZYTaq 2x Green Master Mix

nr kat.: MB36202
Opakowanie: 200 react.
Cena brutto: 369,86 zł 369.86
Cena netto: 300,70 zł
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Producent: NZYTech
nr kat.: MB36202

Opis

Description: Speedy NZYTaq 2× Green Master Mix is a premixed ready-to-use solution containing NZYSpeedy DNA polymerase (MB108), a recombinant DNA polymerase displaying a faster polymerization reaction than any other conventional non-proofreading enzyme. Only 5 seconds are required for the successful synthesis of 1 kb size DNA. The enzyme retains its speed when amplifying fragments up to 2-3 kb. Faster PCR can be further achieved by increasing the primers melting temperature, which increases PCR annealing temperature, thus allowing combining the annealing and extension PCR steps during PCR cycling (see below). The master mix contains dNTPs, reaction buffer and additives at optimal concentrations for the efficient amplification of a wide range of DNA templates. MgCl2 final concentration is 2.5 mM, allowing the implementation of a variety of PCR protocols. In addition, reactions assembled with Speedy NZYTaq 2× Green Master Mix may be directly loaded onto agarose gels. The mix contains two dyes (blue and yellow) that allow monitoring the progress of the electrophoresis.

Resulting PCR products have an A-overhang and are suitable for cloning with NZYTech´s NZY-A PCR cloning kit (MB053) or NZY-A Speedy PCR cloning kit (MB137).

Features:
– Convenient ready-to-use PCR master mix
– Direct Gel Loading
– Fast routine amplification (5 sec/kb)
– High yields
– Leaves an A-overhang

Applications:
– Fast PCR
– Powerful PCR
– Colony PCR
– Generation of products for TA cloning

Specifications:

Product length: 0-2 kb
Hot-start-like capacity: no
Extension time: 5 sec/kb at 72 °C
3´→5´ activity: no
Product overhang: 3’-A
Storage conditions: Store at -20 ºC
Shipping conditions: Shipped at 4 ºC to dry ice

Components:
Speedy NZYTaq 2× Green Master Mix (0.2 U/μL)

Dane techniczne

Opakowanie 200 react.
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