Speedy Ligase (50 react.)
Opis
Speedy Ligase is an improved DNA Ligase developed to carry out fast (less than 15 minutes) and efficient ligation of sticky or blunt-end DNA at room temperature. The enzyme catalyses the formation of a phosphodiester bond between juxtaposed 5′-phosphoryl and 3′-hydroxyl termini in duplex DNA. Rapid ligation is based on the combination of Speedy Ligase with a 4× Speedy ligation buffer.
Features
Fast (5 min for cohesive-ends or 15 min for blunt-ends at room temperature)
Free from detectable nonspecific nuclease, endonuclease, RNase and DNase activities
Supplied with an optimized reaction buffer for efficient ligation reactions
Applications
Cloning of both blunt- and sticky (cohesive)-end restriction fragments
Cloning of PCR products
Joining linkers and adapters to DNA
Nick repair
Self-circularization of linear DNA
Specifications
Enzyme concentration: 10 U/μL
Optimal reaction temperature: room temprature (20-25 °C)
Heat inactivation: 65 °C for 10 min
Storage conditions: Store at -20 °C
Shipping conditions: Shipped at dry ice
Components
Speedy Ligase (10 U/μL)
Reaction buffer (4x)
Dane techniczne
Opakowanie | 50 ligations |