Produkty
Producenci
SensoLyte® ADHP Peroxidase Assay Kit *Fluorimetric*
Opis
HRP conjugates are extensively used as secondary detection reagents in ELISAs, immuno-histochemical techniques and Northern, Southern and Western blot analyses. SensoLyte® ADHP Peroxidase Assay Kit uses highly purified ADHP to quantify peroxidase activity in solutions, in cell extracts and in live cells and on solid surfaces (such as PVDF membranes).
The kit can be used for characterizing kinetics of enzyme reaction and high throughput screening of oxidase inhibitors. The kit contains: ADHP peroxidase substrate (Ex/Em=573/590 nm upon oxidation), Calibration standard, Reaction buffer, Stop buffer, An optimized ‘mix and read’ assay protocol that is compatible with HTS liquid handling instruments
Kits components
Component A: ADHP: 10 mM, 250 µL
Component B: H2O2: 1 vial
Component C: Assay buffer: 60 mL
Absorbance (nm): 573
Emission (nm): 590
Dane techniczne
| Opakowanie | 1 kit |