S288C

A unique collection of knockout strains covering 96% of the yeast genome, containing over 6,000 gene-disruption mutants with molecular barcodes.


By means of two sequential PCR reactions - the first to incorporate the appropriate tags and confer the antibiotic resistance gene and the second to incorporate the mitotic recombination sites - each ORF was replaced with a KanMX cassette using homologous recombination. This method allowed for greater than 95% of the ORFs to be knocked-out.

Disclaimer
We provide certain clone resources developed by leading academic laboratories. Many of these resources address the needs of specialized research communities not served by other commercial entities. In order to provide these as a public resource, we depend on the contributing academic laboratories for quality control. Therefore, these are distributed in the format provided by the contributing institution "as is" with no additional product validation or guarantee. We are not responsible for any errors or performance issues. Additional information can be found in the product manual as well as in associated published articles (if available). Alternatively, the source academic institution can be contacted directly for troubleshooting.

Shipping Information
Individual yeast knock out strains are provided as a live culture in a 2 mL tube. Each tube contains YPD broth with G418 (200 µg/mL) and is supplemented with 15% glycerol. Within 3 to 4 days of receiving your order we will ship your clone at room temperature via express delivery. Store the stock clone at 4°C for up to one week or -80°C indefinitely.

The Yeast Knock Out collections are available as frozen glycerol stocks in 96-well plates. The glycerol stocks are a culture of the Yeast Knock Out in YPD media and glycerol. These plates ship on dry ice and can be maintained indefinitely at -80°C.

References
E. A. Winzeler et al., Functional characterization of the Saccharomyces cerevisiae genome by gene deletion and parallel analysis. Science. 285(5429), 901-906 (6 August 1999).
G. Giaever et al., Functional profiling of the Saccharomyces cerevisiae genome. Nature. 418, 387-391 (2002).
A. Wach, A. Brachat, R. Poehlmann, P. Philippsen, New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae. Yeast. 10(13), 1793-1808 (December 1994).