Kategorie

Producenci

Newsletter

Podaj swój adres e-mail, jeżeli chcesz otrzymywać informacje o nowościach i promocjach.

Recombinant Exonuclease III (> 10 U//uL)

Cena brutto: 285,11 €
Cena netto: 263,99 €
szt.
Opakowanie:
2x 1.25 mL
Kod produktu:

GE-NUC00500-02

Producent:

 

Exonuclease III (ExoIII) of E. coli is a 31 kD monomeric, globular protein combining multiple catalytic activities in one active site. It acts on doublestranded (ds) DNA as a 3'-5' exonuclease, a 3'- phosphomonoesterase, an apurinic/apyrimidimic (AP) sites specific endonuclease and an exonucleolytic ribonuclease H. Of particular interest for molecular biological methods is its exonucleolytic activity, removing 5'-mononucleotides from the 3'-hydroxyl ends of ds DNA, leaving protruding 5'-termini. Its catalytic rate can be adjusted by temperature and NaCl concentration and thus allows for the generation of single-stranded DNA templates for sequencing or recombination methods.


Applications

  • Construction of nested unidirectional deletions of DNA fragments in combination with nuclease S1
  • Generation of a single-stranded template for dideoxy sequencing of DNA
  • Site-directed mutagenesis
  • Cloning of PCR products
  • In vitro recombination

Produkty powiązane

Nazwa produktu / Producent Nr. katalogowy Opakowanie Cena brutto  
DNase I (one Column with 200 µl matrix G3m)
MoBiTec
N3402 88 U
155,47 €
Phusion Site-Directed Mutagenesis Kit
Thermo Scientific (Fermentas, Finnzymes, Pierce, Abgene)
F-541 20 react.
1 001,71 zł

Inni klienci również zamawiali:

Nazwa produktu / Producent Nr. katalogowy Opakowanie Cena brutto  
Ribonuclease H
Thermo Scientific (Fermentas, Finnzymes, Pierce, Abgene)
EN0202 500 U
3 036,62 zł
Deoxyribonuclease I (RNase free), supplied with MnCl2
Thermo Scientific (Fermentas, Finnzymes, Pierce, Abgene)
EN0525 1000 U
332,72 zł
Exonuclease III (Exo III)
Thermo Scientific (Fermentas, Finnzymes, Pierce, Abgene)
EN0191 4000 U
159,53 zł
Recombinant RNase T2 (> 2,5 U/uL)
MoBiTec
GE-NUC00400-01 300 uL
306,89 €
Ribonuclease T1
Thermo Scientific (Fermentas, Finnzymes, Pierce, Abgene)
EN0541 100000 U
332,72 zł
Sklep internetowy Shoper.pl