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QPCR Standard Chromatrap Pro-G high throughput ChIP 96 well Microplate

nr kat.: 500163
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Opis

Chromatrap is a more efficient, sensitive and robust method of ChIP

Compared to standard methods, Chromatrap® is:

  • FASTER
  • EASIER to use
  • MORE SENSITIVE especially for low-abundant targets.

 


Chromatrap® ChIP kits for qPCR rapidly improve ChIP using their unique patented technology streamlining the process, ensuring chromatin preparation to qPCR analysis can be performed in under 5 hours. Without the use of magnetic or agarose beads eliminates non specific background and reduced manual handling error and sample loss.  Optimized elution buffer chemistry allows samples to be analysed directly in qPCR without the need for DNA clean up, further reducing sample loss.

This kit is designed for use as qPCR as the downstream process only and has a wide dynamic range suitable for low chromatin loadings (1µg-10µg). Optimized for 1000ng sample sizes allowing for better results from small sample sizes and provides greater flexibility thus more IP assays can be performed from just one sample.

The kits contain sufficient Protein-A or Protein-G based spin-columns, buffers and reagents to perform 96 chromatin immunoprecipitation assays and up to 10 chromatin preparations by sonication.  An enzymatic shearing format is also available. Premium kits containing positive and negative antibody and primer sets to provide you with satisfaction of chromatin preparation. A high throughput format is also available allowing for multiple sample type, antibody and gene targets to be tested in parallel.

Chromatrap® qPCR kits provide a more convenient, quicker and simpler method for performing ChIP assays in laboratories that do not require sequencing. These kits are cost effective and ideal where qPCR results alone are sufficient.

Chromatrap® qPCR kits outperform all other chromatin immunoprecipitation methods:
 

Robust signal to noise
● Typically 2-3 times better than standard methods

Low non-specific binding of the inert Chromatrap® discs
● No pre-blocking step required
● No DNA clean-up required

High surface area and excellent molecular mixing – ideal for
● Low abundant targets
● Challenging cell types, including primary cells

Excellent ‘flow through’ characteristics of the column
● Easy, fast washing and elution steps during the assay
● Ensures highest levels of chromatin capture and release

Wide dynamic range
● From 50 ng to 7000 ng
● More ChIP assays from a single sample

Reduced incubation steps


What is Chromatrap?

Chromatrap® technology
Chromatrap® kits use spin columns or microplates which contain discs of a revolutionary inert, porous polymer to which protein A or G has been covalently attached. This patented format is unique. During an assay, the chromatin/antibody complex is selectively retained by the disc. Washing with three buffers and an elution step are all that is required to obtain the selectively enriched DNA, making Chromatrap® more efficient in the laboratory.
 

 

What does this mean in the laboratory?

Chromatrap® outperforms other chromatin immunoprecipitation assays because it has:

  • Robust signal to noise, typically 2-3 times better than standard methods
  • Low non-specific binding of the inert Chromatrap® discs
  • No DNA clean-up required for qPCR
  • High surface area and molecular mixing ideal for low abundant targets and challenging cell types.
  • Wide dynamic range make is suitable for ChIPseq
  • 96 well format for high throughput analysis
  • Allows more ChIP assays to be performed from a single sample

 

High surface area and flow-through characteristics are key to efficiency.

 

 

Solid phase scaffold

  • Inert material
  • Open structure
  • Pro A/G bound to surface


Flow through process

  • Promotes molecular movement
  • Better sample mixing
  • Minimises non-specific binding

 


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