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Proteinase K

nr kat.: MB01902
Opakowanie: 100mg
Cena brutto: 1 825,44 zł 1825.44
Cena netto: 1 484,10 zł
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Producent: NZYTech
nr kat.: MB01902

Opis

Proteinase K is a stable serine protease with broad substrate specificity. It degrades many proteins in the native state even in the presence of detergents (1% Triton or 0.5% SDS) and it is used for protolytic inactivation of nucleases during isolation of DNA and RNA. Presented as a white lyophilized powder.

Purity: DNase and RNase none detected

Storage: -20 °C

Activity
≥ 30 U/mg lyophilizate
≥ 40 U/mg protein

One unit of Proteinase K hydrolyses urea- denaturated haemoglobin producing colour equivalent of 1 μmol tyrosine per min at 37 °C and pH 7.5

Preparation instructions
For immediate use: 50 mM Tris-HCl, pH 7.8, 3 mM CaCl2 or water, stable if stored at 4°C up to 1 month.
For long-term storage: 50 mM Tris-HCl, pH 7.8, 3 mM CaCl2, 50% glycerol (v/v), stable if stored at – 20 °C up to 2 years.
The protein solubility is up to 20 mg/ml.
The enzyme is typically used at 50–200 μg/mL in nucleic acid preparations at pH 7.5–8.0 and 37-55 °C. Incubation times vary from 30 minutes to 18 hours.

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