Pierce™ ChIP-grade Protein A/G Magnetic Beads

Description

Efficiently isolate chromatin-bound DNA by immunoprecipitation (chromatin IP) for subsequent quantitation by PCR using Thermo Scientific™ Pierce Magnetic ChIP Kit.

The Pierce Magnetic ChIP Kit contains sufficient reagents to perform 30 chromatin immunoprecipitation (ChIP) assays with appropriate controls using an optimized protocol. The kit includes reagents for cell lysis, capture of protein-DNA complexes, reversal of crosslinking, and DNA isolation. The blocked Pierce Protein A/G Magnetic Beads provide high binding capacity, low non-specific background, and work with many antibody species. These beads can be used manually with a magnetic stand or with automated platforms, such as the Thermo Scientific KingFisher Instruments. ChIP-validated and quality-guaranteed antibodies are also available for use with the Pierce Magnetic ChIP Kit.

Highlights:

• Rapid – obtain purified DNA that is ready for quantitative PCR in about 8 hours

• Efficient and reproducible – micrococcal nuclease digestion and nuclear lysis are highly optimized

• Sensitive – obtain results with as few as 10,000 cells (1 x 10⁴)

• Low background – Pierce Protein A/G Magnetic Beads are blocked in a non-DNA-containing reagent to minimize background

• Complete – optimized positive control reagents are included: RNA polymerase II antibody and GAPDH promoter PCR primers

Includes:

Kit contains reagents for chromatin preparation, IP, DNA purification, and positive controls (antibody and primers)

Requires:

In vivo crosslinker (such as formaldehyde), micro-tip sonicator (such as Misonix™ Sonicator 3000), ChIP qualified antibody of choice, PCR primers for DNA sequence of interest, PCR master mix (containing a dye such as SYBR™ Green, if qPCR is desired), and a qPCR instrument

Recommended for:

• Determine sites of specific protein-DNA interactions on genomic DNA by PCR or ChIP-Seq
• Monitor the effects of histone modifications or chemical agents on DNA binding

Formulation   Magnetite- and protein-coated polymer beads at 10mg/mL in water with sodium azide.