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Producenci

One.Step RT-qPCR with EvaGreen/ROX (2X concentrated)

nr kat.: 105-532
Opakowanie: 1000 x 25 μl rxns
Cena brutto: do wyceny
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Producent: GeneOn
nr kat.: 105-532

Opis

The One.Step RT-PCR Kit is highly sensitive: less than 10 pg to 100 ng total RNA or < 1 pg to 20 ng poly(A) RNA (mRNA) can be detected when using highly expressed transcripts.

Manufactured and quality-controlled in accordance with ISO 9001:2000

Features:
The kit contains all reagents required for RT-qPCR in a set to ensure fast and easy preparation with a minimum of pipetting steps. Just add template, primers. That's all. 

Description:
The enzyme mix is based on a new formulated reverse transcriptase, a Hot-Start Polymerase and RNase Inhibitor. The set is providing increased specificity, high cDNA yield and improved efficiency for highly structured and long cDNA fragments. The reaction buffer includes extrapure dNTPs, EvaGreen as intercalating dye; ROX and reaction enhancers.

Principal:
In the RT-step the reverse transcriptase synthesizes single-stranded DNA molecules (cDNA) complementary to the RNA template. The hot-start polymerase activity is blocked.
In the first cycle of the PCR step the Hot-Start DNA polymerase activity is switched on and it synthesizes DNA molecules complementary to the cDNA, thus generating a double-stranded DNA template. The kit is optimized for all real-time PCR cyclers who are compatible with the evaluation of the ROX reference signal. Select the same optical setting for EvaGreen than for SYBR-Green EvaGreen (excmax= 500 nm; Emimax= 530 nm).

The One.step Realtime RT-PCR Master Mix from GeneON offers a tremendous convenience when applied to analysis of targets from multiple samples of RNA and minimizes the risk of contaminations.

Platforms: The Kit is suitable for all block-based Thermocycler. Stringent Quality Tests on ABI StepOne plus PCR Cycler

Components of Maximo.OneStep RT-qPCR Mix:

  • Enzyme-mix: HotStart Taq Polymerase, Reverse Transcriptase, RNase Inhibitor and enhancers, Reaction buffer, extra pure dNTPs, EvaGreen DNA dye and ROX
  • RNase-free water

 

Protocols / Manuals
The datasheet includes detailed protocols for:

1.) RT-PCR assay without sample denaturation (as a standard RNA/primer combinations)

2.) RT-PCR assay with sample denaturation (RNA/primer with a high degree of secondary
 structure)

2.a) Preparation of RNA / Primer Mix
2.b) Denaturation and primer annealing
2.c) Preparation of the RT-PCR Mix

3.) Reverse Transcription and thermal cycling

 

Storage: @ -20°C, avoid frequent thawing and freezing, store all components with EvaGreen / ROX in the dark

 

Dane techniczne

Opakowanie 1000 x 25 μl rxns
do góry
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