Nucleic Acid Removal Kit
Opis
Nucleic acids significantly interfere with protein purification and analysis in many aspects including cell lysis, protein chromatographic purification, quantification, activity assays and crystallization. The interference is more severe when the interested protein binds DNA or RNA of different sizes. The Nucleic Acid Removal Kit is designed to remove nucleic acids to a level below 250 pg / ml from a protein solution or cell lysate. Each Kit provides reagents for treatment of 200 ml of cell lysate or protein solution.
The nucleic acids are removed by precipitation based on ionic interactions between the nucleic acids and poly cations provided with the kit. The proteins stay in the solution. The precipitation process is completed in 15 minutes and the precipitate is removed by centrifugation or filtration.
Dane techniczne
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