Description: NZYTaq II DNA polymerase belongs to a new generation of Taq-derived DNA polymerases that was engineered to produce high DNA yields in shorter PCR running times (15-30 s/kb extension) under minimal optimization conditions. NZYTaq II DNA polymerase supports the robust and reliable amplification of a wide range of DNA templates up to 6 kb. NZYTaq II is provided with 5× Gel Load Reaction Buffer allowing reactions to be loaded directly into gels without the extra adding of loading dye. This Gel Load Reaction Buffer is composed by a blue and yellow dye. The blue dye migrates at the same rate as a 3-5 kb DNA fragment in a 1% (w/v) agarose gel. The yellow dye migrates at a rate faster than primers (<50 bp) in a 1% (v/v) agarose gel. The 5× Gel Load Reaction Buffer is not suitable when direct fluorescent or absorbance readings are required without prior purification of the amplified DNA from PCR. NZYTaq II DNA polymerase lacks 3´→5´ exonuclease activity. Resulting PCR products have an A-overhang and are suitable for cloning with NZYTech´s NZY-A PCR cloning kit (MB053) or NZY-A Speedy PCR cloning kit (MB137).
Features:
– Suitable for immediate loading onto agarose gels
– Robust amplification using a wide range of DNA templates
– Leaves an A-overhang
Applications:
– Powerful PCR
– Generation of products for TA cloning
Specifications:
Product length: | 0-6 kb |
Hot-start-like capacity: | no |
Extension time: | 15-30 sec/kb at 72 ºC |
3´→5´ activity: | no |
Product overhang: | 3’-A |
Storage conditions: | Store at -20 ºC |
Shipping conditions: | Shipped at 4 ºC to dry ice |
Components:
– NZYTaq II DNA polymerase (5 U/μL)
– Gel Load Reaction buffer (5x)
– MgCl2 Solution (50 mM)