Description: NZYTaq II 2× Colourless Master Mix is a premixed ready-to-use solution containing NZYTaq II DNA polymerase (MB354), which belongs to a new generation of DNA polymerases derived from Taq DNA polymerase with high processivity and robustness. The master mix contains dNTPs and reaction buffer at optimal concentrations for efficient amplification of a wide range of DNA templates up to 6 kb. MgCl2 final concentration is 2.5 mM, allowing the implementation of a comprehensive range of PCR protocols. Resulting PCR products have an A-overhang and are suitable for cloning with NZYTech´s NZY-A PCR cloning kit (MB053) or NZY-A Speedy PCR cloning kit (MB137).
Features:
– Convenient ready-to-use PCR master mix
– High yields with minimal optimization
– Robust amplification using a wide range of DNA templates
– Leaves an A-overhang
Applications:
– Powerful PCR
– Colony PCR
– Generation of products for TA cloning
Specifications:
Product length: | 0-6 kb |
Hot-start-like capacity: | no |
Extension time: | 15-30 sec/kb at 72 °C |
3´→5´ activity: | no |
Product overhang: | 3’-A |
Storage conditions: | Store at -20 ºC |
Shipping conditions: | Shipped at 4 ºC to dry ice |
Components:
NZYTaq II 2× Colourless Master Mix (0.2 U/μL)