Produkty
Producenci
NZYSpeedy Miniprep
Opis
Description:
NZYSpeedy Miniprep kit is designed for the ultra-rapid, small-scale preparation of highly pure plasmid DNA from recombinant Escherichia coli strains. NZYTech´s speedy miniprep procedure is based on the alkaline lysis of bacterial cells followed by adsorption of DNA onto a new specially treated silica membrane in the presence of high salt. The plasmid DNA is selectively adsorbed in the silica gel-based NZYTech plasmid spin column and other impurities such as proteins, salts, nucleotides and oligos (<40-mer) are washed away. The eluted DNA is suitable for applications like automated fluorescent sequencing, PCR and a wide range of other enzymatic manipulations. NZYSpeedy Miniprep kit includes a newly developed washing buffer (AS) which ensures complete removal of contaminants in a rapid washing step.
Features:
– Fast and easy mini-prep of pure plasmid DNA
– Maximum contamination removal in a rapid whashing step with buffer AS
– Spin column silica-based
– High yields of plasmid DNA (15-35 µg of high-copy number plasmid DNA/ column)
– Prevents the use of toxic substances (e.g. phenol, chloroform or CsCl)
Applications:
The purified plasmid DNA is suitable for use in the most demanding molecular biology applications, including:
– Cloning
– Automated fluorescent or manual sequencing
– PCR
– In vitro transcription
– Enzymatic reactions
– Hybridization
– Transfection
High-yield plasmid DNA mini preparation using a rapid protocol:
NZYSpeedy Miniprep provides an efficient and fast way to isolate high yield and high quality plasmid DNA from ≤ 5 mL of an Escherichia coli LB culture.
Plasmid DNA was isolated from 3 mL of a recombinant E. coli strain using three different rapid protocols (NZYTech, lanes 1-4; Competitor 1, lanes 5-8; Competitor 2, lanes 9-12). DNA isolation was performed according to each manufacturer ́s protocol. The quadruplicates (2 μL of 50 μL eluate) were analysed through agarose gel electrophoresis (A). Lane M: NZYDNA Ladder III. Eluates were also analysed by UV/VIS spectroscopy to determine total yields (B).
Specifications:
| Culture volume/starting material: | 1-5 mL E. coli culture volume |
| Plasmid type: | high- and low-copy number, BACs or cosmids |
| Format: | Spin-column |
| Yield: | up to 35 µg |
| A260/A280: | 1.8–2.0 |
| Elution volume: | 30-50 µL |
| Binding capacity: | 35 µg |
| Procedure: | Manual – centrifugation |
| Preparation time: | ≤ 12 min |
| Product size: | 50, 200, 2×50 and 5×200 columns |
| Storage conditions: | All kit components can be stored at room temperature; Buffer A1 with RNase should be stored at 4 °C for frequent use |
| Shipping conditions: | Shipped at room temperature |
Components:
– Buffer A1
– Buffer A2
– Buffer A3
– Buffer AS (concentrate)
– Buffer AE (does not contain EDTA)
– RNase A
– NZYTech Spin Columns
– Collection Tubes (2 mL)
Dane techniczne
| Opakowanie | 2x 50 preps. |