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NZYLong 2x Colourless Master Mix

nr kat.: MB33201
Opakowanie: 100 react
Cena brutto: 279,21 zł 279.21
Cena netto: 227,00 zł
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Producent: NZYTech
nr kat.: MB33201

Opis

Description: NZYLong 2× Colourless Master Mix is a premixed ready-to-use solution containing NZYLong DNA polymerase (MB003), an engineered DNA polymerase designed to amplify long target DNA sequences, generally of 20 kb and beyond. A wide range of long PCR products can be generated using lambda DNA or human genomic DNA as starting template. The master mix contains dNTPs, reaction buffer and additives at optimal concentrations for the efficient amplification of an extensive range of DNA templates. MgCl2 final concentration is 2.5 mM, allowing the implementation of a variety of PCR protocols.

NZYLong DNA polymerase generates a mixture of A-overhang-ended (predominantly) and blunt-ended PCR products, being suitable for cloning with NZYTech´s TA PCR cloning kits (MB053 or MB137).

Features:
– Convenient ready-to-use PCR master mix
– Amplifies long DNA fragments (up to 20 kDa)
– Higher fidelity than NZYTaq DNA polymerase

Applications:
– Long-Range PCR
– Colony PCR
– Generation of products for TA cloning

Specifications:

Product length: 0-20 kb
Hot-start-like capacity: no
Extension time: 60 sec/kb at 68 °C
3´→5´ activity: no
Product overhang: mixed
Storage conditions: Store at -20 ºC
Shipping conditions: Shipped at 4 ºC to dry ice

Components:
NZYLong 2× Colourless Master Mix (0.2 U/μL)

Dane techniczne

Opakowanie 100 react
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