Multiplex PCR NZYTaq 2x Green Master Mix

Description: Multiplex PCR NZYTaq 2× Green Master Mix is a premixed ready-to-use solution designed for the simultaneous amplification of multiple DNA fragments (up to 15 targets) in a single tube. The Master Mix contains dNTPs and reaction buffer at optimal concentrations for efficient multiplexing of targets ranging in size from 70 bp to 2.5 kb over a broad range of primer and template concentrations. MgCl₂ final concentration is 2.5 mM, allowing the implementation of a comprehensive variety of PCR protocols. Amplification efficiency is further improved by the inclusion of a recombinant modified enzyme derived from Taq DNA polymerase, which was engineered to display a hot-start-like PCR capacity and high processivity (fast polymerisation). The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer-dimers, and thus ensuring highly specific and sensitive multiplex PCR amplification. In addition, reactions assembled with Multiplex PCR NZYTaq 2× Green Master Mix may be directly loaded onto agarose gels, which simplifies the workflow. There are two dyes (blue and yellow) in the mix that allow monitoring the progress of electrophoresis.

Features:
– High performance in multiplexing up to 15 primer pairs
– Direct Gel Loading
– High sensitivity and specificity
– Minimal optimization requirements
– Leaves an A-overhang

Applications:
– Multiplex PCR
– PCR
– Typing/ Detection
– Generation of products for TA cloning

Efficient Multiplex PCR with low amounts of template
Comparison of PCR performance using Multiplex PCR NZYTaq 2x Green Master Mix or a similar competitor product in a multiplex reaction to amplify 10 different-sized fragments (250-1200 bp) from a dilution-series of human genomic DNA.

Both multiplex PCR mixes show similar sensitivity, resulting in specific amplification with significant high yields of all targets, even with less amount of template. M: DNA Ladder.

Specifications:

Components:
Multiplex PCR NZYTaq 2× Green Master Mix (0.2 U/μL)