MulV First-Strand cDNA Synthesis Kit, separate oligo

Description: NZY M-MuLV First-Strand cDNA Synthesis Kit, separate oligos, is a system that includes all the necessary components to synthesize first-strand cDNA, except the template RNA. Random hexamers and Oligo(dT)18 primers are provided in separate tubes to offer the convenience to choose the appropriate primer to initiate your reverse transcription reaction.

The resulting single-stranded cDNA is suitable for use in rea-ltime quantitative Reverse Transcription PCR (RT-qPCR). NZY M-MuLV First-Strand cDNA Synthesis Kit, separate oligos, is formulated to provide high yields of full-length cDNA products and to increase sensitivity in RT-qPCR.

Starting material can range from 10 pg up to 5 µg of total RNA. Besides random hexamers and Oligo(dT)18 primers, the kit includes NZYRT 2× Master Mix, no oligos, which contains dNTPs, MgCl2 and an optimized RT buffer; 10× Annealing Buffer and NZYM-MuLV RT Enzyme Mix. NZYM-MuLV RT Enzyme Mix includes both NZY M-MuLV Reverse Transcriptase (RNase H minus) and NZY Ribonuclease Inhibitor in order to protect RNA against degradation due to ribonuclease contamination. RNase H (from E. coli) is provided in a separate tube to specifically degrade the RNA template in cDNA:RNA hybrids after the first-strand cDNA synthesis. This procedure will improve the sensitivity of subsequent RT-qPCR reactions since PCR primers will bind more easily to the cDNA.

Features

• Possibility to choose the primer to initiate the reaction
• Cost-effective kit using NZY M-MuLV Reverse Transcriptase
• Provides high yields of full-length cDNA products
• Formulated to increase sensitivity in RT-qPCR
• Starting material: 10 pg to 5 µg of total RNA
• Optimal reaction temperature: 37 °C
• Convenient and reliable

Applications

• First-strand cDNA synthesis for use in real-time quantitative RT-PCR (RT-qPCR)
• Two-step RT-PCR assays

Components

• NZYM-MuLV RT Enzyme Mix
• NZYRT 2x Master Mix, no oligos
• 10× Annealing Buffer
• Random hexamer mix (50 ng/µL)
• Oligo(dT)18 primer mix (50 µM)
• NZY RNase H (E. coli)
• DEPC-treated H2O