Mobicol Gravi Fixed with Luer-Lock and screw cap, fixed outlet plug, without filters

The Versatile Small Column with Inserted Filter for Affinity Chromatography

Features

• Can be used in flow-through mode or as spin column
• Useful for size exclusion or affinity purification of compounds like nucleic acids and proteins (native or tagged)
• Compatible with small and large sample volumes
• Luer-lock cap is compatible to Luer syringes
• Screw cap with rubber seal
• Mobicol can be packed and stored or shipped without leakage
• Reverse side of snap-off plug can be used for resealing
• Empty columns for other filter pore sizes available
• Luer adapters are available for inlet and outlet tubing
• Autoclavable (with filters at 110 °C) for 10 min
• Can be centrifuged in standard microcentrifuge
• Materials: polypropylene (column corpus), polyethylene (filters)

Mobicol “F” (order# M105035F) with fixed snap-off plug for the outlet is supplied with 2 caps: a Luer-lock cap and a screw cap. The Mobicol “F” can be closed tightly with screw cap and plug. A filter of 35 µm pore size is inserted; an additional upper filter (35 µm) is available.

The Mobicol “F” columns for affinity chromatography are compatible to the laboratory standard. With affinity matrix they are convenient for:

• Purification of tagged proteins
• Purification of peptides
• Purification of native proteins using immobilized IgG, NTP-binding proteins (e.g. kinases), amino acid binding proteins, ß-lactamase, Igase, peroxidase, EPO (erythropoetin), ß-galactosidase
• Fractionation and purification of antibodies using protein A/G, immobilized epitopes, peptide matrix
• Purification of glycoproteins and carbohydrates
• Purification of fatty acids and fatty acid binding proteins
• Whole mount embryo in situ hybridization

Syringe connected to Luer-lock cap (left) and adapter (order# M3001) tubing to Mobicol.

The Mobicol "F" columns are available pre-packed with, e.g., immobilized proteases, nucleases, alk. phosphatase, ß-galactosidase, or a compound of your choice on request.
Column accessories like tubings or Luer adapters are separately available, please see