Minute™ Protein and/or Nucleic Acid Extraction Kit from Gels
Opis
Passive elution and electro elution are the most common methods for extracting proteins and/or nucleic acid from polyacrylamide/agarose gels. Protein extraction by passive elution usually takes overnight incubation. Eluted proteins are significantly diluted and require further concentration. Protein extraction by electro elution is faster (30 min to 2 h) than passive elution but it requires a special elution device that works less effectively for larger proteins (>70 Kda). The electro buffer usually contains detergent and other chemicals at a concentration that may interfere with downstream applications. Here we feature a rapid and instrument-free protein/nucleic acid extraction kit. Proteins/nucleic acid can be extracted from gels in <10 min with high yield. The elution buffer can be a detergent-containing buffer or pure water depending upon methods of gel staining. The elution volume is between 10 to 200 μl. Multiple gel pieces can be processed in a single tube and the final protein concentration is relatively high.
Application
Extracted proteins can be used for MALD-MS analysis, immunization of animals, protein-protein interaction and protein-nucleic acid interaction studies etc. Extracted nucleic acid can be used for PCR, cloning, and sequencing etc.
Pre-stained protein markers (15 µg/lane) were separated in 10% SDS-PAGE.
Protiein bands from two lanes were excised and extracted as described in the protocol. Proteins in the gels were eluted with 40 µl ddH2O. Eluted Proteins were separated in 10% SDS-PAGE without concentration. 10 µl pre-stained protein marker was loaded in this figure.
Kit components
1. 20 filter cartridges with 2.0 ml collection tubes
2. 20 microfuge tubes (200 μl)
3. 2 gram extraction powder
4. Micro pestle X 4
Dane techniczne
Opakowanie | 20 tests |