Maximo M-SuperhotTaq DNA Polymerase (qPCR)
Opis
Features
Maximo M-Superhot Taq DNA Polymerase for qPCR is designed for Real-Time PCR and Hot-start PCR. A special inhibitor suppresses the reaction at room temperature until after the first denaturation step. This prevents primer-dimers and other artefacts. Using the enzyme there is no need to adjust the existing standard PCR protocol.
Applications
- Hot Start and real time PCR
- Multiplex PCR
- Amplification of complex genomic and cDNA templates
- No primer-dimers and other artêfacts; inactive at room temperature
- Short activation time for real time PCR
- Enhanced PCR sensitivity
Description
Maximo M-Superhot Taq DNA for qPCR and Hot-Start-PCR is an optimized mixture of a high processive Taq DNA Polymerase and special inhibitors to Taq DNA for real time PCR. The enzyme is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a double-stranded specific 5´→3´ exonuclease activity. The enzyme consists of a single polypeptide with a molecular weight of 94kDa. It is developed for real time PCR or as basis enzyme for real time PCR diagnostics systems.
Concentration: 5 u/µl
Unit definition
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP's into acid-insoluble form in 30 minutes at 74oC under assay conditions:
25mM TAPS pH 9.3 at 25oC, 50mM KCl, 2mM MgCl2; 1mM beta-mercaptoethanol; 200µM each dATP, dGTP, dTTP and 100 µM dCTP (a mix of unlabled and µ-[32P]-labled); 12.5 µg activated salmon sperm DNA in the final volume of 50 µl
Storage Buffer
20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.1 mM EDTA; 1 mM DTT, 50 % Glycerol, 0.5 % Nonident P-40, 0.5 % Tween-20
Reaction Buffer
Reaction buffer (10X)” incomplete” (red cap):160 mM (NH4)2SO4, 670mM TrisHCl pH8,8, 0,1% Tween-20
Reaction buffer (10X) “complete” (yellow cap):160 mM (NH4)2SO4, 670mM TrisHCl pH8,8, 0,1% Tween-20, 25mM MgCl2
separate Tube: MgCl2 (100 mM, green cap)
Quality control
Activity and performance test in real time PCR, SDS-PAGE purity, absence of endonucleases/nickases and exonucleases test.
Usage
Use your existing and optimized protocol. In contrast to chemically modified Taq DNA pol. where the first denaturation step needs up to 15 min, Maximo M-Superhot Taq for Real Time PCR does not need a prolonged heating or denaturation time and works excellent basis enzyme for real time PCR.
Dane techniczne
Opakowanie | 5000 U |