Produkty
Producenci
M-MuLV Reverse Transcriptase
Opis
MMLV-Reverse Transcriptase: The enzyme that synthesizes a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single stranded DNA as a template.
Applications
- RT PCR
- Synthesis of cDNA
- mRNA 5’-end Mapping by Primer Extension Analysis
- End-labeling of DNA
- Dideoxynucleotide Sequencing
Description
MMLV Reverse Transcriptase, encoded by Moloney Murine Leukemia Virus (MMLV RT) is an RNA-dependent DNA polymerase that synthesizes the cDNA first strand from a single-stranded RNA template to which a primer has been hybridized.MMLV RT will also extend primers hybridized to single-stranded DNA. Second strand cDNA synthesis can be achieved from some mRNA templates without an additional DNA polymerase.
Concentration: 200 u/µl
Storage Buffer
200 mM potassium phosphate (pH 7.2), 0.2% Triton X-100, 2 mM DTT and 50% glycerol
Reaction Buffer 5X
250 mM Tris-HC1(pH8.3), 375 mM KCl, 15 mM MgCl2 and 50 mM DTT
Dane techniczne
| Opakowanie | 50000 U |