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Luminaris Color HiGreen qPCR Master Mix

nr kat.: K0394
Opakowanie: 5000 react.
Cena brutto: do wyceny
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Opis

 

Thermo Scientific Luminaris Color HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.
The Hot Start Taq DNA polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1). The Luminaris Color HiGreen qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.
The use of Luminaris Color HiGreen qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color HiGreen qPCR Master Mixes with ROX at different concentrations or fluorescein as passive reference dyes are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights

  • Blue master mix and Yellow Sample Buffer for easy pipetting
  • UDG in the master mix to prevent carry-over contamination
  • Excellent qPCR performance:

 

- Specificity – Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers

- Sensitivity – detects low copy number targets

- Wide linear range – accurate quantification across 9 orders of magnitude

- Reproducibility and convenience – ready-to-use 2X master mix

  • Versions with premixed ROX or fluorescein for different real-time platforms
  • Standard cycling protocols

Applications

  • Gene expression analysis
  • siRNA validation
  • Genotyping
  • Pathogen detection

Includes

  • Luminaris Color HiGreen qPCR Master Mixes include Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye
  • 40X Yellow Sample Buffer with a yellow dye
  • Nuclease-free water

 

Human c-Myc gene was amplified with the Luminaris Color HiGreen qPCR Master Mix on the PikoReal 96 Real-Time PCR System using cDNA reverse transcribed from HeLa cells total RNA (10 ng – 0.1 pg) by Maxima First Strand cDNA Synthesis Kit for RT-qPCR (Cat. #K1641). The insert shows the melting curve analysis results indicating high specificity of the amplification. Reaction efficiency E = 98.1%; R2 = 0.9984

Luminaris Color qPCR Master Mixes (2X) contain a blue dye. The 40X Yellow Sample Buffer is included as a separate vial. This buffer can be mixed with the samples to provide visual aid when pipetting samples.

White reaction vessels are especially good for qPCR applications, as they deliver higher signal intensities than clear vessels. However, traditional colorless reaction components are poorly visible in white wells, making reaction setup more difficult. Luminaris Color qPCR Master Mixes overcome this difficulty and decrease the risk of pipetting errors. In the image, the blue wells contain only the 2X Master Mix. The green color indicates the sample DNA with yellow color has also been added into the reaction.

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Tabelka kompatybilności zestawów Luminaris z aparatami do qPCR.  

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