Low Molecular Weight (LMW) Protein Marker II

The Low Molecular Weight Protein Marker II for SDS electrophoresis is a liquid mixture of eight purified proteins for use in molecular weight estimation in the presence of the detergent sodium dodecyl sulphate (SDS). This protein marker contains no oligosaccharides that may cause anomalous migrations and heterogeneous fuzzy bands. The molecular mass of the protein under investigation is determined by comparing its electrophoretic mobility with that of proteins contained in the kit. Three (MB21401) or six (MB21402) 500 µL vials are supplied, each containing a mixture of highly purified protein standards of molecular mass ranging from 11000 to 96000 when used in denaturing polyacrylamide electrophoresis. The mixture is supplied in a loading buffer for direct loading on gels.

Features:
– Ready-to-load form
– Precisely defined size intervals between bands

Applications:
– Estimation of protein molecular weight by SDS-PAGE

Specifications:
– Size Range: 11 – 96 kDa
– Stain type: Unstained
– Storage conditions: Store at -20 °C
– Shipping conditions: Shipped at 4 ºC

Components:
– 3 x 500 µL (300 applications of 5 µL each) or 6 x 500 µL (600 applications of 5 µL each) provided in loading buffer consisting of 30% (w/v) glycerol, 2% (w/v) SDS, 25 mM NaHepes, pH 7.5, 50 mM DTT, 5 mM EDTA, 0.02% (w/v) NaN3

Recommendations for loading
1. Thaw the marker at 37-40 °C for a few minutes. An initial precipitate is normal due to SDS.
2. Mix gently, but thoroughly, to ensure that the solution is homogeneous and clear.
4. Heat this tube at 95 °C for 5 minutes to completely denature proteins.
5. Chill on ice.
6. Make 50 μLaliquots and keep them at –20 °C while not in use (additional heating is not required; for next uses, simply thaw the aliquots at 37-40 °C for a few minutes and mix until the solution is homogeneous and clear).
7. Load the following volumes of the marker on SDS-PAGE:
-5 μL per well for mini-gels;
-10 μL per well for large gels