Klenow Fragment of DNA Polymerase I
Opis
Klenow Fragment of DNA Polymerase I is a truncated fragment of E. coli DNA polymerase I that lacks the 5′ → 3′ exonuclease activity while retaining the 3′ → 5′ exonuclease activity (proofreading).
Features
- Free from detectable RNase and DNase activities
- Flexible for a range of molecular applications
- Can incorporate modified nucleotides (e.g. biotin, digoxigenin, fluorescently, labeled nucleotides)
- Supplied with an optimized reaction buffer for efficient activity
Applications
- 3´-end labelling of DNA fragments for sequence analysis
- Conversion of 5´-overhangs to create blunt-ends for cloning
- Removal of 3´-overhangs
- Second-strand synthesis of cDNA
- Random primer labelling of DNA fragments
Specifications
- Enzyme concentration: 5 U/μL
- Source: Recombinant E. coli strain
- Optimal reaction temperature: 37 °C
- Heat inactivation: 75 °C for 10 min
- Storage conditions: Store at -20 °C
- Shipping conditions: Shipped at dry ice
Dane techniczne
Opakowanie | 300 U |