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Klenow Fragment of DNA Polymerase I

nr kat.: MB00901
Opakowanie: 300 U
Cena brutto: 275,64 zł 275.64
Cena netto: 224,10 zł
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Producent: NZYTech
nr kat.: MB00901

Opis

Klenow Fragment of DNA Polymerase I is a truncated fragment of E. coli DNA polymerase I that lacks the 5′ → 3′ exonuclease activity while retaining the 3′ → 5′ exonuclease activity (proofreading).

Features

  • Free from detectable RNase and DNase activities
  • Flexible for a range of molecular applications
  • Can incorporate modified nucleotides (e.g. biotin, digoxigenin, fluorescently, labeled nucleotides)
  • Supplied with an optimized reaction buffer for efficient activity


Applications

  • 3´-end labelling of DNA fragments for sequence analysis
  • Conversion of 5´-overhangs to create blunt-ends for cloning
  • Removal of 3´-overhangs
  • Second-strand synthesis of cDNA
  • Random primer labelling of DNA fragments


Specifications

  • Enzyme concentration: 5 U/μL
  • Source:    Recombinant E. coli strain
  • Optimal reaction temperature: 37 °C
  • Heat inactivation: 75 °C for 10 min
  • Storage conditions: Store at -20 °C
  • Shipping conditions: Shipped at dry ice

Dane techniczne

Opakowanie 300 U

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