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Producenci

Kex2 Protease (Lys/Arg-Arg), recombinant

nr kat.: EP0450
Opakowanie: 50 U
Cena brutto: do wyceny
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Opis

  • Specific serine endoproteinase
  • Cleaves amino acid sequence N-Arg-Arg/-C and N-Lys-Arg/-C at the carboxyl end
  • Suited for protein sequencing and cleavage of fusion proteins with an appropriate recognition sequence

 

In  the  series  of  endoproteinases  we  also  offer  the  newly  developed  endoproteinase  Lys/Arg-Arg.  The specific serine endoproteinase (universal serine-protease) has a MW of 68 kDa. It cleaves at the carboxyl end of the recognition sequences: Arg-Arg/X and Lys-Arg/X and thus provides new possibilities for e.g. the development of fusion protein systems with alternative cleavage sites.


Technical Details

Source: Saccharomyces cerevisiae

Activators: Endoproteinase  Lys/Arg-Arg is  strongly Ca2+dependent and has a pH-optimum at pH 7.0

Inhibitors: Ala-Lys-Arg-chloro-methyl ketone

Note: Endoproteinase  Lys/Arg-Arg  isnot  inhibited  by  phenyl-methyl-sulfonyl-fluoride and tosyl-lysine-chloro-methylketone.

Reaction buffer: 50 mM Tris-HCl or HEPES, 5 mM CaCl2, pH 7.0 (0.5 mM PMSF; 0.1%Triton  X-100  is  not  included,  but  might be  added  if  required  by  yourapplication).  For  fusion  proteins  the  reaction  conditions  have  to  bedetermined empirically.

Substrate solution: Benzyloxycarbonyl-L-tyrosyl-L-lysyl-L-arginin-4-nitroanilid (Z-L-Tyr-Lys-Arg-pNA ◦ TFA salt, Bachem  No.  L1250).  The  substrate is dissolved in reaction buffer  (OD315=  12).  This  solution  can  be stored at -20°C for several months. Thaw at room temperature immediately before use.

Unit definition: 1 unit endoproteinase Lys/Arg-Arg releases 1 μmole 4-nitroaniline perminute in reaction buffer at pH 7.0 at RT.

Specific activity: 2.2 U/ml

Dane techniczne

Opakowanie 50 U

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