GeneJET FFPE DNA Purification Kit

Formalin-fixed and paraffin-embedded (FFPE) tissue samples are routinely prepared from human surgical tissue samples allowing to store them in archives for years. The Thermo Scientific GeneJET FFPE DNA Purification Kit is designed for fast and convenient purification of DNA from various amounts of archived FFPE tissue. Up to 8 sections (10 µm thickness) of FFPE sample from various tissues can be used for genomic DNA extraction without overnight incubation. Elimination of toxic reagents commonly used for deparaffinization (i.e., xylene) allows a safe DNA purification procedure with high DNA yields and excellent quality. Each preparation recovers up to 8 µg of genomic DNA that can be eluted in volumes from 20 µL allowing for concentrated samples. Due to high decrosslinking efficiency high quality purified DNA shows reliable performance in sensitive downstream applications such as qPCR, PCR (amplicons up to 1 kb) or NGS library preparation.

Highlights

• High quality DNA – suitable for PCR and qPCR
• Fast – procedure can be completed within 2 hours
• Safe - non toxic solvents
• Efficient - yields up to 8 µg of purified DNA

Applications

• PCR
• qPCR
• NGS library preparation

Yields of genomic DNA purified from one section (10 µm of thickness) of various human FFPE tissues using the GeneJET FFPE DNA Purification Kit

 Includes

• Proteinase K Solution
• RNase A Solution
• Digestion Buffer
• Binding Buffer
• Wash Buffer I (conc.)
• Wash Buffer II (conc.)
• Elution Buffer
• GeneJET DNA Purification Columns & Collection Tubes
• Collection Tubes
• Detailed Protocol