G418 Disulfate Solution (50 mg/ml in Water)

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CAS Number: 108321-42-2
Molecular Formula: C₂₀H₄₀N₄O₁₀ · 2H₂SO₄
Molecular Weight: 692.71 g/mol
Form: Solution
Appearance: Clear colorless solution
Solubility: Solution
Source: Biosynthetic: produced by Micromonospora rhodorangea.
pH: 4.6-6.0
Optical Rotation: +104° to +121°
Storage Conditions: 28°C

Description
G418 disulfate is an aminoglycoside antibiotic related to the gentamicins, especially gentamicin B1. G418 disulfate is most commonly used as a gene selection agent in cell culture transfection procedures to isolate G418 resistant eukaryotic cells.
*The product names G418 disulfate and G418 sulfate are synonyms and can be used interchangably.
TOKU-E also offers G418 disulfate powder (G001) and G418 disulfate, EvoPure (G030) (≥99.0% pure).

Mechanism
G418 disulfate prevents protein synthesis by blocking the elongation step in prokaryotic and eukaryotic ribosomes. Spectrum: G418 disulfate is toxic to susceptible prokaryotic and eukaryotic cells including fungi (yeasts and molds), bacteria, mammalian and plant cells.

Microbiology Applications
G418 disulfate can be used as a selection agent for G418 resistant bacteria or fungi after transformation.

Mammalian Biology Applications
G418 disulfate is routinely used as a selection agent in cell culture after transfection of eukaryotic cells. G418 resistant cells express the neo gene which produces aminoglycoside 3'-phosphotransferase (APT 3' II) - a protein that confers resistance to G418 and other aminoglycoside antibiotics.
For more information on relevant cell lines, culture medium, and working concentrations, please visit the TOKU-E Cell-culture Database. G418 disulfate 50mg/ml in water.

References
Aragão F.J.L. and Brasileiro A.C.M., Positive, negative and markerfree strategies for transgenic plant selection. Braz. J. Plant Physiol., 14(1):1-10, 2002
Davis, Bernard D. "Mechanism of Bactericidal Action of Aminoglycosides."Microbiological Reviews 51.3 (1987): 341-50.
Dong Z.J. and McHughen, A. Improved procedure for production of transgenic flax plants using Agrobacterium tumefaciens. Plant Science, 88 (1993) 61-71 61. Elsevier Scientific Publishers.