First.Strand cDNA PLUS Synthesis Kit (20 µl / reaction)

Manufactured and quality-controlled in accordance with ISO 9001:2000

 
• For synthesis of cDNA from 100 bp up to 10 kb length
• Higher cDNA yield and greater efficiency for full length cDNA synthesis compared with standard M-MLV RT
• Increased thermostability

Description:
The First Strand cDNA Plus Synthesis kit contains all reagents for first strand cDNA synthesis and offers a simple handling with high flexibility. A genetically engineered version of M-MLV Reverse Transcriptase with no RNase H activity is a RNA-directed DNA enzyme that synthesizes a complementary DNA strand initiating from a primer using single-stranded RNA or DNA as template.

Applications:

• Synthesis of highly structured and long cDNAfragments
• Extremely sensitive and highly specific RTPCR
• DNA labeling

Components:

• Reverse Transcriptase (200 U / µl)
• 5 X Complete RT Buffer
• dNTP Mix (10 mM each)
• DTT stock solution (100 mM)
• Oligo-(dT)20 primer (100 µM)
• Random Hexamers (100 µM)
• RNase Inhibitor (40 U / µl)
• RNase-free Water

QC-Tests:
 - Purified free of endo- and exodeoxyribonucleases, phosphatases and ribonuclease
 - Activity and stability tested in first strand cDNA synthesis.

Protocols:

Note:
For many standard combinations of RNA and primers heat treatment may be omitted with no negative effect on results. A sample denaturation is particularly recommended for RNA targets that:
 - exhibit a high degree of secondary structure
 - self- or cross-complementary primers
 - initial experiments with new targets

Storage:
Store product @ -20°C. Avoid frequent thawing and freezing