First.Strand cDNA PLUS Synthesis Kit (20 µl / reaction)
Opis
Manufactured and quality-controlled in accordance with ISO 9001:2000
• For synthesis of cDNA from 100 bp up to 10 kb length
• Higher cDNA yield and greater efficiency for full length cDNA synthesis compared with standard M-MLV RT
• Increased thermostability
The First Strand cDNA Plus Synthesis kit contains all reagents for first strand cDNA synthesis and offers a simple handling with high flexibility. A genetically engineered version of M-MLV Reverse Transcriptase with no RNase H activity is a RNA-directed DNA enzyme that synthesizes a complementary DNA strand initiating from a primer using single-stranded RNA or DNA as template.
Applications:
- Synthesis of highly structured and long cDNAfragments
- Extremely sensitive and highly specific RTPCR
- DNA labeling
Components:
- Reverse Transcriptase (200 U / µl)
- 5 X Complete RT Buffer
- dNTP Mix (10 mM each)
- DTT stock solution (100 mM)
- Oligo-(dT)20 primer (100 µM)
- Random Hexamers (100 µM)
- RNase Inhibitor (40 U / µl)
- RNase-free Water
QC-Tests:
- Purified free of endo- and exodeoxyribonucleases, phosphatases and ribonuclease
- Activity and stability tested in first strand cDNA synthesis.
Protocols:
Note:
For many standard combinations of RNA and primers heat treatment may be omitted with no negative effect on results. A sample denaturation is particularly recommended for RNA targets that:
- exhibit a high degree of secondary structure
- self- or cross-complementary primers
- initial experiments with new targets
Storage:
Store product @ -20°C. Avoid frequent thawing and freezing
Dane techniczne
Opakowanie | 20 react. |