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FastGene apTaq HotStart Polymerase

Cena brutto: 489,35 zł
Cena netto: 453,10 zł
szt.
Opakowanie:
500U
Kod produktu:

LS34

Producent:
Pliki do pobrania:

DNA-Polymerase with aptamer technology: Reversible enzyme activation

Advantages

  •     Hot start: Very fast PCR
  •     Aptamer technology: Reversible enzyme activation or inactivation
  •     Maximal specificity, sensitivity and yield
  •     Robust and reliable reaction
  •     Tolerate a wide range of templates



apTaq HotStart Polymerase – Redefine your PCR

The FastGene® apTaq DNA-Polymerase is a recombinant and thermostable Taq-Polymerase using the aptamer based Hot Start activation technology. The aptamer allows a reversible and immediate activation of the polymerase, leading to specific priming and a very fast PCR.

 
Reversible Polymerase activation – The aptamer principle

The FastGene® apTaq DNA-Polymerase is a Hot Start enzyme, which is completely inactive during room-temperature and become active only after heating. Random primer annealing and unspecific amplification that can occur with standard PCR enzymes are problems of yesterday. In contrast to antibody-based methods, the apTaq DNA-Polymerase contains synthetically manufactured aptamer-oligonucleotides.

At low temperatures the polymerase is inactivated through a reversible tight bond of the aptamer. Interestingly, the aptamer acts as a molecular switch, changing its tertiary structure at higher temperatures. Temperatures below 45° C inactive the polymerase, whereas temperatures above 60 °C fully activated the enzyme. Therefore, the FastGene® apTaq DNA-Polymerase is less temperature-sensitive and reduce the risk of contamination.

 

Schematic explanation of the aptamer principle



The polymerase aptamer-oligonucleotide mixture is a reversible, temperature-dependent Hot Start system.

 
Applications

The FastGene® apTaq HotStart Polymerase is ideally suited for:

  •     Fast PCR
  •     Routine PCR
  •     PCR using complex templates
  •     SNP Analysis
  •     Any standard PCR application for which a hot start formulation of a high-quality thermostable DNA polymerase is required

Inni klienci również zamawiali:

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Phire Green Hot Start II DNA Polymerase
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Phusion U Hot Start DNA Polymerase
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F-555S 100 U
Phusion U Green Hot Start DNA Polymerase
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F-556S 100 U
FastGene® HotStart TAQ Polymerase
Nippon
LS23 100 U
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