FastGene Western ECL Kit
Opis
Chemiluminescent substrate based on luminol for high femtogram or low picogram detection
Key Benefits
- High sensitivity – Detection of antigens with very low concentrations
- Long signal duration – Optimal for digital and film-based imaging
- No optimization required – Easy switch from other brands
- Perfect for PDVF and nitrocellulose membranes
- Compatible with Western Blotting Markers
FastGene® Western ECL Kit – Chemiluminescent Western Blot Detection
The FastGene® Western ECL Kit is an enhanced chemiluminescent substrate based on luminol.
It is used to detect horseradish peroxidase (HRP) – conjugated secondary antibodies.
The high femtogram or low picogram detection of antigen is enabled by FastGene® Western ECL Kit brilliant sensitivity and long signal duration.
This long chemiluminescent signal duration makes it possible to detect signals on both digital and film-based imaging systems without any loss in signal intensity.
Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration.
Workflow using FastGene® Western ECL Kit
Mix the luminol solution and peroxide solution in a 1:1 ratio, and thoroughly agitate the chemiluminescent substrate solution well for preparing the 0.1 ml of solution / cm2 of membrane.
Place the membrane with the protein side up and remove the membrane from the chemiluminescent substrate solution. Image the membrane with a digital imager.
Comparison between the FastGene® Western ECL Kit with 3 competitor products
All ECL kits were used under the same experimental workflow.
An IL-6 fusion protein was detected using a primary antibody against IL-6 from mouse and a secondary anti-mouse peroxide (POD) antibody 2 seconds exposure for the Western Blot.
Contains:
50 ml Peroxide solution
50 ml Luminol Solution
Dane techniczne
Opakowanie | 100 ml |