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FastGene AflII

nr kat.: FG-AflII
Opakowanie: 500 react.
Cena brutto: 286,34 zł 286.34
Cena netto: 232,80 zł
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Producent: Nippon
nr kat.: FG-AflII

Opis

  • No Star Activity
  • Highest Purity
  • Restriction in just 5 minutes with the FastCut buffer
  • Perfect use for Cloning experiments

 

Overhang CpG Methylation
5′ TTAA Nicht sensitiv
Incubation Temp. Heat Inactivation
37°C 65°C for 20 min

Activity in FastGene Puffer

Buffer I Buffer II Buffer III Buffer IV FastCut Puffer
75% 100% 75% 100% 100%
Supplied Buffer Enzyme Type
Buffer IV Type IIP
Isoschizomers
BfrI, BspTI, BstAFI, MspCI, Vha464I
Recognition Sequence Amount
C/TTAAG
GAATT/C 
2000 units, 4 units/µl

ENZYME FINDER


Different Types of restriction enzymes

Based on the structure, cofactor requirements and specificity of cleavage there are four types of restriction enzymes (Types I, II, III, and IV).

Type I restriction enzymes cleaves the DNA at a random location far away from the recognition sequence. These enzymes require both ATP and S-adenosyl-L-methionine to function.

Type II restriction enzymes cleaves the DNA within or near the recognition sequence. These enzymes do not require ATP and are independent from methylase. The type II enzymes are the most useful restriction endonucleases for the daily laboratory work. All our NIPPON Genetics EUROPE restriction enzymes are from type II.

Type III restriction enzymes cleaves DNA about 20 – 25 base pairs away from the recognition sequence. They require both ATP and S-adenosyl-L-methionine to function.

Type IV restriction enzymes cleaves only modified, typically methylated DNA in contrast to the types I-III, which are usually inhibited by methylation.

 

Ambiguity Codes

N = A or C or G or T (any)
B = C or G or T (not A)
D = A or G or T (not C)
H = A or C or T (not G)
V = A or C or G (not T)
W = A or T (weak)
S = C or G (strong)
R = A or G (purine)
Y = C or T (pyrimidine)
M = A or C (amino)
K = G or T (keto)

Dane techniczne

Opakowanie 500 react.

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