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Equine Genotypes Panel 1.1

nr kat.: F-850S
Opakowanie: 100 react.
Cena brutto: do wyceny
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Opis

Parentage testing and individual identification using short tandem repeat (STR) loci Short Tandem Repeat (STR) loci, or microsatellites, are a class of nuclear DNA markers consisting of tandemly repeated sequence motifs of two to seven base pairs in length. Alleles of STR loci vary by the number of times a given sequence motif is repeated. STR alleles are detected using Polymerase
Chain Reaction (PCR) and by separating the amplification products using electrophoresis.
Due to their high level of polymorphism (informativeness) and Mendelian inheritance, microsatellites have become the markers of choice for parentage testing and individual identification.

The Thermo Scientific Equine Genotypes Panel 1.1 encompasses the following 17 STR loci: VHL20, HTG4, AHT4, HMS7, HTG6, AHT5, HMS6, ASB23, ASB2, HTG10, HTG7, HMS3, HMS2, ASB17, LEX3, HMS1 and CA425 (Table 1). These include nine loci recommended by the ‘Equine Genetics and Thoroughbred

Parentage Testing
Standardization Committee’ of the International Society for Animal Genetics (ISAG) and eight additional loci commonly used for horse parentage testing and identification.
The Equine Genotypes™ Panel 1.1 allows co-amplification of the 17 microsatellites in a single multiplex PCR reaction. One primer from each primer pair is end-labeled with a fluorescent dye. After PCR, the fragments are separated and detected in a single electrophoresis injection, using an automated electrophoresis instrument, such as ABI PRISM® 310 Genetic Analyzer or ABI PRISM 3100 Genetic Analyzer (both Applied Biosystems).

The Equine Genotypes Panel 1.1 provides all the necessary reagents for amplification of the 17 microsatellite loci. In addition, the kit includes equine control DNA for verifying acceptable PCR and electrophoresis conditions.

Kit performance characteristics
The Equine Genotypes Panel 1.1 delivers optimal results when 1–2 nanograms of high-quality genomic DNA is applied in the PCR reaction volume of 20 μL. The reagents and reaction protocols of the Equine Genotypes Panel 1.1 have been optimized to deliver similar amplification yields (peak sizes) for alleles within and among loci, when an appropriate amount of high-quality DNA is applied.
The kit uses Thermo Scientific Phusion Hot Start DNA Polymerase. Allele callings obtained with this kit represent the true alleles of an individual, instead of ‘plus-A’ peaks or ‘split peaks’ typically interpreted when using a conventional Taq DNA polymerase. This is due to the proofreading (3'→5' exonuclease) activity of the PhusionTM Hot Start DNA Polymerase. The results are not impaired by the tendency of DNA polymerases to add an extra nucleotide (most often adenine) to the end of the amplification products.

Protocol

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