DreamTaq™ Hot Start Green PCR Master Mix
Opis
Thermo Scientific™ DreamTaq™ Hot Start Green PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq™ Hot Start DNA Polymerase, DreamTaq Green buffer, magnesium, and dNTPs. The master mix allows easy reaction setup with fewer pipetting steps, with only primers, template, and water needing to be added. The green master mix includes density reagent and two tracking dyes for convenient direct loading of PCR products on a gel.
DreamTaq Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases.
DreamTaq Hot Start DNA Polymerase employs antibody-based inhibition of DNA polymerase activity at ambient temperatures to prevent the amplification of non-specific products prior to the amplification step. With DreamTaq Hot Start DNA Polymerase, reactions can be set up at room temperature using the same protocol and cycling conditions as conventional Taq DNA polymerases.
DreamTaq Hot Start Green PCR Master Mix is formulated to enhance productivity through:
- Better reaction outcomes
--Higher yield of target amplicons from low template amounts
--Increased reaction specificity due to reliable hot-start technology
--Wider range of amplicon lengths—routine amplification of genomic DNA fragments up to 6 kb
- Enhanced convenience
--Room temperature reaction set-up
--Minimized optimization of Mg2+ concentration and of primer annealing temperatures due to optimized reaction buffer
--Simplified workflow and minimized pipetting steps with 2X master mix format
--Direct loading of PCR products on a gel
Applications:
- Routine PCR
- Colony PCR
- Genotyping
- RT-PCR
- Generation of PCR products for TA cloning
Features:
- Hot Start: Built-In Hot Start
- GC-Rich PCR Performance: Low
- Product Overhang: 3'-A
- Reaction Speed: Standard
- Shipping Condition: Shipment on Wet or Dry Ice
DreamTaq Hot Start formats
EP1701 DreamTaq™ Hot Start DNA Polymerase 200 U
EP1702 DreamTaq™ Hot Start DNA Polymerase 500 U
EP1703 DreamTaq™ Hot Start DNA Polymerase 2500 U
EP1704 DreamTaq™ Hot Start DNA Polymerase 4 x 2500 U
K9011 DreamTaq™ Hot Start PCR Master Mix 200 rxns
K9012 DreamTaq™ Hot Start PCR Master Mix 1000 rxns
DreamTaq Hot Start Green formats
EP1711 DreamTaq™ Hot Start Green DNA Polymerase 200 U
EP1712 DreamTaq™ Hot Start Green DNA Polymerase 500 U
EP1713 DreamTaq™ Hot Start Green DNA Polymerase 2500 U
EP1714 DreamTaq™ Hot Start Green DNA Polymerase 4 x 2500 U
K9021 DreamTaq™ Hot Start Green PCR Master Mix 200 rxns
K9022 DreamTaq™ Hot Start Green PCR Master Mix 1000 rxns
Dane techniczne
Opakowanie | 200 react. |