DNAreleasy Advance

Direct PCR - Lysis reagent

Go from cells to PCR in less than 15 mins!

Nippon Genetics is proud to introduce a novel reagent specially formulated in order to eliminate nucleic acid purification for PCR analysis.

DNAreleasy Advance is a continuous development of our well know lysis reagent DNAreleasy suitable for template preparation of PCR experiments. DNAreleasy Advance replaces time consuming and tedious extractionand purification methods. The released DNA can be usde directly in a PCR reaction or can be stored at -20°C for several months.

So far DNAreleasy has been tested on various cell types, including:

• Saliva
• Hair roots
• Animal tissue (horse, pig liver etc.)
• Various plant (cabbage, maize, soja, sugar beet, canola)
• Drosophila
• Yeast
• Mollusca

Quick Guide:

1. Cells or tissue is mixed with 30 μl DNAreleasy Advance in a PCR tube. The sample must be covered completely by DNAreleasy Advance.
2. The tube will be transfered to a thermal cycler and the following lysis conditions will be performed: Step 1: 65°C for 5 minutes; Step 2: 96°C for 5 minutes; Step 3: 20°C for 5 minutes.
3. For  the beginning we recommend to use different volumes of the lysate into the PCR reaction, in order to estimate the best working condition.

DNAreleasy Advance is a complex mixture of different components which will result in a release of genomic DNA. Neverhteless we need to mention that this procedure is more an extraction as a purification and therefore the released DNA can't be used in photometer assays in order to determine the concentration and purity of the DNA.
DNAreleasy Advance can be used for RESEARCH ONLY.

Genomic DNA from scallops was isolated by DNAreleasy Advance and a part of the supernatant was directly added to the PCR reaction. The agarose gel shows the high yield of amplified DNA fragment.
Data kindly provided by Dr. Schubbert, Eurofins Medigenomix GmbH, Ebersberg, Germany.