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DNA Loading (6X) Buffer for Agarose and Acrylamide Gels WYCOFANY

nr kat.: 306-205
Opakowanie: 5x1 ml
Cena brutto: do wyceny
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Producent: GeneOn
nr kat.: 306-205

Opis

WYCOFANY. ZAMIENNIK: 306-210

 

The loading dye increases the density of the sample and they add colour to the sample, thereby simplifying the loading process. The solution contains dyes that, in a electric field, move toward the anode at predictable rates.

In 1% agarose gels, bromophenol blue migrates with 300 bp linear double-stranded DNA fragment, whereas xylene cyanol FF migrates at approximately the same rate as linear double-stranded DNA 4 kb length. These relationships are not significantly affected by the concentration (0.5 to 1.4%) of agarose in the gel.

Loading: The loading dye suits well for the DNA samples dissolved either in water or in EDTA-containing buffer (as TE buffer). If DNA markers are not prediluted with the Loading dye solution, then mix: The loading buffer is 6x concentrated, that means you have to use it 5:1.
For DNA markers, apply 0.1 µg per 1 mm of agarose gel lane width. Often 1µg of marker is used in one electrophoresis run but it depends on the size of your gel and the comb.

Preparation: 6x Loading Dye Solution deionised water at a ratio 1:1:4 for example, 5 µl 1 kb ladder : 5 µl 6x loading dye : 20 µl water. By applying 30.0 µl of this mixture, you’ll have 1.0 µg of total DNA per lane.

Storage and transport: @ RT or -20 °C.

R0611

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Opakowanie 5x1 ml
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