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Calcein; AM *UltraPure Grade* *Small Package*

nr kat.: AS-89204
Opakowanie: 20 x 50 µg
Cena brutto: do wyceny
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Producent: Anaspec
nr kat.: AS-89204

Opis

Purity: ≥95% by HPLC
Spectral Properties: Abs/Em = 494/517 nm
Solvent System: DMSO

Calcein, AM is a cell-permeant and non-fluorescent compound that is widely used for determining cell viability. In live cells the non-fluorescent calcein, AM is hydrolyzed by intracellular esterases into the strongly green fluorescent anion calcein. The fluorescent calcein is well-retained in the cytoplasm in live cells. Hela cells stained with AnaSpec’s Calcein AM fluorescent dye (Cat# 89201, 89202, 89203 and 89204).

MCF-7 cells grown in 8-well slide chamber for 2 days. Cells were washed twice before adding 1 uM Calcein AM (AnaSpec cat# 89202). Images acquired every 3 seconds and total elapsed time on video is 12 min.

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Opakowanie 20 x 50 µg
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