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CSM-URA (POWDER)

nr kat.: 114511212
Opakowanie: 10 g
Cena brutto: do wyceny
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Producent: MP Biomedicals
nr kat.: 114511212

Opis

Single dropout formulation ( without Uracil) of Complete Supplement Mixture (CSM) of amino acids for S. cerevisiae growth media.

Application Notes     

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. This CSM-Dropout medium create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

Formulation Details     

Contents per Liter:

Adenine 10* mg |L-Arginine HCl 50 mg |L-Aspartic Acid 80 mg |L-Histidine HCl 20 mg |L-Isoleucine 50 mg |L-Leucine 100 mg |L-Lysine HCl 50mg |L-Methionine 20** mg |L-Phenylalanine 50 mg |L-Threonine 100** mg |L-Tryptophan 50 mg |Uracil 0 mg| L-Tyrosine 50 mg |L-Valine 140 mg |Total 770.000 |*Minimum quantity for healthy growth and yet optimized to promote red color in certain adenine auxotrophs. CSM formulations are available that contain 20 or 40 mg/liter adenine. **80mg/liter of DL-Homoserine is substituted for L-Threonine in mixtures where L-Methionine is dropped out.

Grade: Molecular Biology Certified™

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Opakowanie 10 g
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